On the afternoon of September 18, at the invitation of Professor Kenichi Tsuda of our college, Dr. Michitaka Notaguchi, Associate Professor of Nagoya University, Japan, and made an online academic report entitled Study on the Mechanism of Grafting on the 93rd high-end forum of plant protection. More than 100 teachers and students participated in the online report which was presided over by Professor Tsuda.
Professor Notaguchi said that grafting is a common asexual reproduction technology, but the incompatibility of distant grafting is a bottleneck in agricultural production. He found that the Solanum plant Nicotiana benthamiana can be grafted with many other families. The interfamily grafting interface of Nicotiana benthamiana or Arabidopsis thaliana can be connected by callus to realize the intercommunication and material circulation between the apoplast and the symplasm. By comparative transcriptome analysis of the junctions between affinity and disaffinity families, it was found that some gene expressions related to cell wall were significantly up-regulated with the occurrence of grafting, indicating the importance of cell wall reconstruction for the success of interfamily grafting. The key protein β-1,4-glucanase (GH9B3), which determines the success of grafting, was further identified. Its cellulase activity can promote the cell wall reconstruction of the grafting interface and realize the interfamily grafting of Nicotiana benthamiana. Furthermore, β-1,4-glucanase also plays an important role in the interaction between parasitic plants and hosts. Finally, Professor Notaguchi also showed that the micro-graft chip designed by the research group can greatly improve the maneuverability of grafting.
After the report, Professor Notaguchi and the teachers and students participating in the report had an in-depth exchange and discussion on the contents of the report.
Dr. Michitaka Notaguchi, Associate Professor of BioFunctional Utilization Research Centre/Life Agronomy Research, Nagoya University, Japan and the Director of GRA&GREEN, is mainly engaged in plant grafting and systemic signal transduction mechanism, as well as the use of grafting to explore mRNA long-distance signal transduction in plants. Many articles have been published in Science、Science Advances and Communications Biology in recent years.